This study was performed to investigate the toxicity of ochratoain A (OA) to the chromosomes of K_(562) tumor cell-line in vitro.
The results of this experiment were as follows:
1) Chromosomes of K_(562) tumor cell-line resulted in pseudotriploidy on the control group. Chromosomes of K_(562) tumor cell-line treated with OA resulted in heteroploidy compared with the control group.
The mean number of chromosomes in the karyotype of the control group (60) were 7 in the A group, 5 in the B group, 20 in the C＋X group, 7 in the D group, 9 in the E group, 6 in the F group, and 6 in the G＋Y group respectively.
The number of chromosomes were increased as follows:
Treating with 0.7μM OA, the number of chromosomes were increased one in E and F group, two in G＋Y group compared with control group. In treated with 1.5μM OA, the increasing number of chromosome was one in E and F group. In treated with 3μM OA, E and F group was increased one and G＋Y group were increased two chromosomes compared with control group. But in treated with 6μM OA, the number of chromosome in G＋Y group was decreased one.
2) K_(562) tumor cell line treated with OA showed Philadelphia-Chromosome in the long arm of the G group karyotype chromosome. The rate of chromosome aberration in K_(562) tumor cell-line treated with OA was 77% in 0.7μM OA group, 71% in 1.5μM OA group, 82% in 3μM OA group and 94% in 6μM OA group respectively. The rate of chromosome aberration of K_(562) tumor cell-line treated with OA was high in the high dose level of OA, and chromosome aberration of K_(562) tumor cell-line treated with OA showed deletion, minute, dicentric-chromosome and translocation in the long arm of the C-group karyotype.
As a result of this study, the toxicity of OA showed deletion, minute, dicentric-chromosome and translocation in the long arm of the C-group karyotype, and then, the toxicity of OA resulted in the damage to RNA and protein synthesis in K_(562) tumor cell-line, and the C-group karyotype of K_(562) tumor cell-line was target of the toxicity of OA.
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